ABSTRACT
ABSTRACT:In recent years ,there has been high prevalence of murine typhus in Yunnan Province ,People's Republic of China .A large outbreak of murine typhus occurred in Xishuangbanna Prefecture ,Yunnan Province in 2010 .However ,not all cases were confirmed by laboratory assays ;therefore ,field epidemiologic and laboratory investigations of murine typhus in Xishuangbanna Prefecture were conducted in 2011 .Blood samples were collected from clinical diagnostic cases at the acute and convalescence stages of murine typhus in Xishuangbanna Prefecture ,Yunnan Province ,from June to September of 2011 ,and blood and spleen samples were collected from mice sharing the same habitats as the patients .Immunofluorescence assays were used to test for the presence of IgM and IgG antibodies against Rickettsia typhi in sera from patients and mice .Real‐time PCR was used to detect the groEL gene of R .typhi in blood clots from patients at the acute stage and in spleen tissue from mice .A total of 1 157 clinically diagnosed murine typhus cases occurred in Xishuangbanna Prefecture ,Yunnan Province in 2011 ,with an incidence of 102 .10/100 000 .Of these cases ,80 were investigated by laboratory assays and 74 of 80 patients were confirmed to have murine typhus .The coincidence rate between the clinical diagnosis and laboratory detection was 92 .50% .The positivi‐ty rate for IgG antibodies against R .typhi was 14 .0% (14/100) for Rattus f lavipectus ,while the rate by PCR was 9 .0%(9/100) .That laboratory diagnoses confirmed that the severity of the murine typhus outbreak in Xishuangbanna cannot be ig‐nored .The distribution of host animals transmitting R .typhi underscores this conclusion .
ABSTRACT
Las queratomicosis por hongos filamentosos son una de las causas de daño en la córnea en los países de climas tropicales y subtropicales y se consideran dentro de las micosis de difícil tratamiento. El presente estudio evalúa la etiología de las queratitis sicóticas en Tucumán (R. Argentina) para determinar su incidencia e importancia clínica regional. En un lapso de 5 años se estudiaron 48 muestras (biopsias, raspados cornéales y/o aspirados oculares) recogidas por el oftalmólogo y enviadas al laboratorio para análisis micológico. Mediante examen directo, cultivos y estudios macro y micromorfológicos se confirmó etiología micótica en 13 pacientes (27 por ciento). De ellos, se identificaron 7 cultivos como Fusarium solani complex, 4 F. oxysporum y 2 F. verticillioides. Estos hallazgos permiten profundizar el conocimiento de los agentes etiológicos locales involucrados y los factores de riesgo, dos aspectos importantes en la prevención y la terapéutica de estas micosis.
Keratomycosis caused by filamentous fungi is one of the agents of damage to the cornea in subtropical and tropical climate countries and belongs to those mycoses identified as of difficult treatment. This study evaluates the etiology of mycotic keratitis in Tucumán (R. Argentina) with the purpose of assessing its incidence and regional and clinical significance. In a 5-year period, 48 samples (biopsy, corneal scrapes and/or ocular aspiration) collected by the oculist were examined and sent to the laboratory for a mycological analysis. By means of direct exam, macro and micromorphological cultures and studies of the presence of mycotic etiology in 13 patients (27 por ciento) was confirmed. Among them 7 cultures such as a Fusarium solani complex, 4 F. oxysporum and 2 F. verticillioides were identified. These findings allow to enlarge the knowledge of the local etiological agents involved as well as the risk factors, two elements that are significant in the prevention and therapeutics of these mycoses.